The intensity of each protein group in a given sample is divided by the median of the
intensities of all protein groups in that sample and then multiplied by the mean of
median of sum of intensities of all protein groups in all samples.
Usage
medianNorm(se, ain = "raw", aout = "Median", on_raw = TRUE)
Arguments
- se
SummarizedExperiment containing all necessary information of the proteomic dataset
- ain
String which assay should be used as input
- aout
String which assay should be used to save normalized data
- on_raw
Boolean specifying whether normalization should be performed on raw or log2-scaled data
Value
SummarizedExperiment containing the median normalized data as assay (on log2 scale)
Examples
data(tuberculosis_TMT_se)
tuberculosis_TMT_se <- medianNorm(tuberculosis_TMT_se, ain = "raw",
aout = "Median", on_raw = TRUE)